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液胞型ATPase(V_0V_1)の構造解析
http://hdl.handle.net/10965/00002925
http://hdl.handle.net/10965/00002925c5af29ca-0467-47d5-a621-8f3724aa9698
名前 / ファイル | ライセンス | アクション |
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KJ00010262459.pdf (279.3 kB)
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Item type | [ELS]紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2018-01-28 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | 液胞型ATPase(V_0V_1)の構造解析 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Structural Studies of the Vacuolar type ATPase | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 液胞型 ATPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 構造解析 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | V-ATPase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | V_0V_1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 電子顕微鏡 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | V-ATPase | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Rotary molecular motor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Structural study | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cryo-electron micrography | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | V_0V_1 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11879037 | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P | |||||
著者 |
横山, 謙
× 横山, 謙× 中西, 温子× 岸川, 淳一 |
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著者所属(日) | ||||||
京都産業大学総合生命科学部 | ||||||
著者所属(日) | ||||||
京都産業大学リエゾンオフィス | ||||||
著者所属(日) | ||||||
京都産業大学リエゾンオフィス | ||||||
記事種別(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 研究論文 | |||||
記事種別(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Papers | |||||
抄録(日) | ||||||
内容記述タイプ | Other | |||||
内容記述 | 液胞型ATPase V_0V_1およびその可溶性部分V_0V_1の構造決定を試みた。V_0V_1全体およびV_1部分のX線結晶構造解析用の結晶を作成した。高分解能の反射データを得るために,現在結晶を大きくするなどの条件検討を行っている。平行して凍結電子顕微鏡(クライオEM)を用いたV₁およびV_0V_1の単粒子解析を行った。V_1に関しては,良好な単粒子像が得られ,構造解析ソフトrelionで解析した結果,~9Å分解能の三次元構造を得た。また,界面活性剤を検討することで,解析に適したV_0V_1の画像が得られた。十分な枚数の画像を撮影し,いまだ明らかにされていないV_0V_1の原子分解能構造を決定する。 | |||||
抄録(英) | ||||||
内容記述タイプ | Other | |||||
内容記述 | Vacuolar type ATPases(V_0V_1)are widely distributed in organisms and function as a proton pump responsible for acidification of intracellular compartments such as a lysosome, Golgi apparatus, endosome so on. In this study we have attempted to determine the structure of V_0V_1 and V_1 moiety. We obtained crystal of both the V_0V_1 and V_1 in several conditions. We are now carrying out the optimization of these crystal conditions. In addition, we observed the molecules of V_0V_1 and V_1 by cryo electron microscopy. Single particle analysis of the V_1 revealed 3-D averaged image at ~9 Å resolution. Also we obtained 2-D averaged images of V_0V_1 in which two stalks structure was clearly observed. These structural analysis of the V_0V_1 and V_1 would shed light on the molecular mechanism of V-ATPases in near future. | |||||
書誌情報 |
京都産業大学総合学術研究所所報 巻 11, p. 99-104, 発行日 2016-07 |
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表示順 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 6 | |||||
アクセション番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | KJ00010262459 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1348-8465 |