{"created":"2023-05-15T14:41:38.169428+00:00","id":1576,"links":{},"metadata":{"_buckets":{"deposit":"99661ec9-5b4b-4bbd-a22f-9086edbbc652"},"_deposit":{"created_by":3,"id":"1576","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1576"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00001576","sets":["14:13:59"]},"author_link":["3956"],"control_number":"1576","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"85","bibliographicPageStart":"60","bibliographicVolumeNumber":"33","bibliographic_titles":[{"bibliographic_title":"京都産業大学論集. 自然科学系列"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The initial step of mucin-type O-glycosylation is catalyzed by UDP-GalNAc : polypeptide N-acetylgalactosaminyltransferases (GalNAc-transferases). GalNAc-transferases contain a lectin domain at the C-terminus, consisting of three tandem repeats (α, β, and γ). I have reevaluated the role of the GalNAc-T1 lectin domain in this study, because the putative lectin domain of this enzyme is reportedly not functional. Deletion of the lectin domain resulted in a complete loss of enzymatic activity. I found that GalNAc-T1 has two activities distinguished by their sensitivities to inhibition with free GalNAc; one activity is sensitive, the other is resistant. In my experiments, the former activity is represented by the O-glycosylation of apomucin, an acceptor that contains multiple glycosylation sites, and the latter by synthetic peptides that contain a single glycosylation site. Site-directed mutagenesis of the lectin domain selectively reduced the follow-up activity, and identified Asp444 in the α repeat as the most important site for GalNAc recognition. I also found that the β repeat recognizes GalNAc and is involved in glycosylation of acceptors with multiple glycosylation sites. These results indicate that the lectin domain of GalNAc-T1 has at least two functional repeats, allowing the possibility of multivalent interactions with GalNAc residues on the acceptor peptide during glycosylation.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"京都産業大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11923897","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-3323","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"天野, 麻理","creatorNameLang":"ja"},{"creatorName":"TENNO, Mari","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-30"}],"displaytype":"detail","filename":"AHSUSK_NSS_33_60.pdf","filesize":[{"value":"924.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AHSUSK_NSS_33_60.pdf","url":"https://ksu.repo.nii.ac.jp/record/1576/files/AHSUSK_NSS_33_60.pdf"},"version_id":"bf0a05c9-ccbc-4e63-82a1-85bbacd4adae"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ムチン型O-グリコシレーション，GalNAc 転移酵素，レクチン様ドメイン，構造活性相関，部位特異的変異","subitem_subject_scheme":"Other"},{"subitem_subject":"mucin-type O-glycosylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"GalNAc-transferase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"lectin domain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"structurefunction relationship","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"site-directed mutagenesis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"UDP-GalNAc :  Polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) の構造活性相関 : GalNAc-T1のレクチン様ドメインの機能解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"UDP-GalNAc :  Polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) の構造活性相関 : GalNAc-T1のレクチン様ドメインの機能解析","subitem_title_language":"ja"},{"subitem_title":"Structure-function Relationship of UDP-GalNAc :  Polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) : Function of the Lectin Domain of GalNAc-T1","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["59"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-30"},"publish_date":"2017-09-30","publish_status":"0","recid":"1576","relation_version_is_last":true,"title":["UDP-GalNAc :  Polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1) の構造活性相関 : GalNAc-T1のレクチン様ドメインの機能解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-22T02:05:48.674459+00:00"}