{"created":"2023-05-15T14:41:39.061626+00:00","id":1597,"links":{},"metadata":{"_buckets":{"deposit":"ab6fa339-2830-42e7-bb67-dac7c1e0cf38"},"_deposit":{"created_by":3,"id":"1597","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1597"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00001597","sets":["14:13:62"]},"author_link":["14814","4035","19370"],"control_number":"1597","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"70","bibliographicPageStart":"59","bibliographicVolumeNumber":"37","bibliographic_titles":[{"bibliographic_title":"京都産業大学論集. 自然科学系列"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ムチン型O–グリコシド型糖鎖はタンパク質の主要な翻訳後修飾の一つであり，UDP-GalNAc : polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) は，その生合成開始反応を触媒する．筆者らはこれまでに，脳・神経特異的に発現するGalNAc-T9および，それと非常に高い相同性をもつGalNAc-T様遺伝子（putative GalNAc-T，以降pt-GalNAc-Tと略）をクローニングした．pt-GalNAc-Tについては，ノーザンブロット解析，in situ hybridization 解析により，それがGalNAc-T9同様，脳特異的に発現することを見いだしている．pt-GalNAc-Tが典型的なムチン型ペプチドに対して糖転移活性を示すことから，pt-GalNAc-TはGalNAc-Tファミリーの新規アイソザイムとみなすことができるので，GalNAc-T16と呼ぶことにする．筆者らは，GalNAc-T9および-T16の生体内での機能を詳細に解析するため，胚性腫瘍細胞P19を用いた．P19はレチノイン酸(RA)存在下で神経に分化する細胞であり，神経分化モデル細胞として様々な研究に用いられている．まず，P19細胞におけるGalNAc-Tファミリーの発現を解析したところ，神経特異的に発現するGalNAc-T9,-T13,-T16の発現が神経分化に伴って亢進することを見いだした．そこで，P19細胞においてこれらの遺伝子の発現を抑制し，神経分化に対する影響を解析した．興味深いことに，GalNAc-T16の発現を抑制したP19細胞においてのみ，神経細胞への分化が見られず細胞死を引き起こした．筆者らはさらに，カスパーゼ解析，FACS解析により，GalNAc-T16ノックダウン細胞における神経分化誘導時にアポトーシスを起こしていることを見いだした．以上の結果から，GalNAc-T16は神経分化において重要な機能を持つと考えられた．","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"1．序論\n2．材料と方法\n3．結果\n4．考察\n参考文献","subitem_description_type":"Other"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"京都産業大学"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11923897","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-3323","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鳥羽, 慎也","creatorNameLang":"ja"},{"creatorName":"トバ, シンヤ","creatorNameLang":"ja-Kana"},{"creatorName":"TOBA, Shinya","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高田, 慎一","creatorNameLang":"ja"},{"creatorName":"タカダ, シンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKADA, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒坂, 光","creatorNameLang":"ja"},{"creatorName":"クロサカ, アキラ","creatorNameLang":"ja-Kana"},{"creatorName":"KUROSAKA, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-30"}],"displaytype":"detail","filename":"AHSUSK_NSS_37_59.pdf","filesize":[{"value":"410.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AHSUSK_NSS_37_59.pdf","url":"https://ksu.repo.nii.ac.jp/record/1597/files/AHSUSK_NSS_37_59.pdf"},"version_id":"a314431a-ffec-4e63-bf93-4afd63f19f25"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"糖鎖修飾","subitem_subject_scheme":"Other"},{"subitem_subject":"ムチン型糖鎖","subitem_subject_scheme":"Other"},{"subitem_subject":"神経分化","subitem_subject_scheme":"Other"},{"subitem_subject":"アポトーシス","subitem_subject_scheme":"Other"},{"subitem_subject":"RNAi","subitem_subject_scheme":"Other"},{"subitem_subject":"glycosylation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mucin-type O-glycan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neural development","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"apoptosis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RNAi","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"UDP-GalNAc : polypeptide N-acetylgalactosaminyltransferase 16の発現抑制によって引き起こされる細胞死に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"UDP-GalNAc : polypeptide N-acetylgalactosaminyltransferase 16の発現抑制によって引き起こされる細胞死に関する研究","subitem_title_language":"ja"},{"subitem_title":"Studies on Apoptosis Caused by the Suppression of UDP-GalNAc : Polypeptide N-acetylgalactosaminyltransferase 16","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"3","path":["62"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-30"},"publish_date":"2017-09-30","publish_status":"0","recid":"1597","relation_version_is_last":true,"title":["UDP-GalNAc : polypeptide N-acetylgalactosaminyltransferase 16の発現抑制によって引き起こされる細胞死に関する研究"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-08-22T00:50:54.920683+00:00"}