{"created":"2024-05-13T02:47:17.519043+00:00","id":2000192,"links":{},"metadata":{"_buckets":{"deposit":"7281cece-1a65-47e7-bc0d-141df846f8c5"},"_deposit":{"created_by":11,"id":"2000192","owner":"11","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2000192"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:02000192","sets":["14:226:1715563349919"]},"author_link":["19368","22828","22665","22829","22666","6251"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-03-29","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"54","bibliographicPageStart":"41","bibliographicVolumeNumber":"18","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"進行性大腸がんは標準治療薬である抗がん剤に薬剤耐性を獲得していることから，完治が難しい。我々は以前の大腸がん培養細胞を用いた研究から，大腸がんが抗がん剤耐性を獲得する原因の1 つとして，線維芽細胞増殖因子受容体3 の選択的スプライシングアイソフォームであるFGFR3IIIc の発現が上昇する可能性を提唱した。本研究では，大腸がん患者臨床検体からオルガノイドを作製し，標準治療薬であるフルオロウラシル（5-FU）に対する耐性とFGFR3IIIc の発現との関係を解析した。大腸がんオルガノイドの免疫組織染色によって，上皮系細胞マーカー上皮細胞接着因子（EpCAM）陽性，細胞増殖マーカーKi67，がんマーカーがん胎児性抗原（CEA）陽性の細胞にFGFR3IIIc の発現が認められた。オルガノイドから抽出したmRNA を用いてRT-PCR によってFGFR3IIIc ともう一つのアイソフォームFGFR3IIIb の発現を解析した。FGFR3IIIb とFGFR3IIIc 発現のハウスキーピング遺伝子GAPDH に対する相対値と，それぞれのオルガノイドの5-FU に対する薬剤感受性IC50 値との相関性を検証した。その結果，正常上皮に発現するFGFR3IIIb と5-FU に対するIC50値の関係は相関関数-0.481 で負の値を示したが，大腸がん細胞に発現するFGFR3IIIc と5-FU に対するIC50 値の関係は相関関数0.057 で正の値を示した。これらの結果から，FGFR3IIIc の発現上昇と5-FU への薬剤耐性獲得に関連が見られることが示唆された。","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"京都産業大学総合学術研究所"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SEO, Misuzu","creatorNameLang":"en"},{"creatorName":"瀬尾, 美鈴","creatorNameLang":"ja"},{"creatorName":"セオ, 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Yoshihisa","creatorNameLang":"en"}],"familyNames":[{"familyName":"奥知","familyNameLang":"ja"},{"familyName":"オクチ","familyNameLang":"ja-Kana"},{"familyName":"OKUCHI","familyNameLang":"en"}],"givenNames":[{"givenName":"慶久","givenNameLang":"ja"},{"givenName":"ヨシヒサ","givenNameLang":"ja-Kana"},{"givenName":"Yoshihisa","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"22829","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"UEDA, Shugo","creatorNameLang":"en"},{"creatorName":"上田, 修吾","creatorNameLang":"ja"},{"creatorName":"ウエダ, 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ノブヒロ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"UENO","familyNameLang":"en"},{"familyName":"上野","familyNameLang":"ja"},{"familyName":"ウエノ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Nobuhiro","givenNameLang":"en"},{"givenName":"信洋","givenNameLang":"ja"},{"givenName":"ノブヒロ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"6251","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-05-13"}],"displaytype":"detail","filename":"KSUSGKS_18_41.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","url":{"label":"KSUSGKS_18_41.pdf","url":"https://ksu.repo.nii.ac.jp/record/2000192/files/KSUSGKS_18_41.pdf"},"version_id":"677663d8-8092-40e5-bc7c-d3309d435605"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"線維芽細胞増殖因子受容体3IIIc","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"大腸がんオルガノイド","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"上皮細胞接着因子（EpCAM）","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"がん胎児性抗原（CEA）","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"抗がん剤耐性","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"フルオロウラシル","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP assay","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Fibroblast growth factor receptor 3IIIc","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"patients-derived colorectal cancer stem organoids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"colorectal cancer marker carcinoembryonic antigen","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"anticancer drug resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fluorouracil","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ATP assay","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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