{"created":"2023-05-15T14:42:22.560163+00:00","id":2641,"links":{},"metadata":{"_buckets":{"deposit":"0554ad91-d0af-407d-8bd1-f4977d38b9d7"},"_deposit":{"created_by":3,"id":"2641","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2641"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002641","sets":["14:179:193"]},"author_link":["21676","21672"],"control_number":"2641","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"40","bibliographicPageStart":"31","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"京都産業大学先端科学技術研究所所報"},{"bibliographic_title":"The bulletin of the Research Institute of Advanced Technology Kyoto Sangyo University","bibliographic_titleLang":"en"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"ミツバチ科マルハナバチに属するエゾオオマルハナバチBombus hypocrita sapporoensisは、特定外来種であるセイヨウオオマルハナバチの代替花粉交配用昆虫として注目されている。今回我われは、本種の農業利用を進めるための遺伝育種学的解析に必要なマイクロサテライトDNAマーカーの適用性を検討した。120種類のマーカーのうち、57種類が多型解析に利用できることがわかった。さらに8種類のプライマーは、マルチプレックスPCRによる同時解析が可能であることを明らかにした。これらのツールキットを利用して女王蜂の受精嚢内から単離した精子DNAの解析を行ったところ、交配雄蜂の遺伝子型を特定できることがわかった。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"The Bumblebee is a eusocial Hymenoptera with an annual life cycle and is often utilized as an agricultural pollinator. We developed an polymorphic microsatellite DNA toolkit for the Japanese bumblebee Bombus hypocrita sapporoensis using multiplex PCR. At 57 of these 120 loci, high allelic variation was observed in 20 individual males. In addition to a method of spermathecae PCR, we developed 8 polymorphic microsatellite DNA toolkit for the B. h. sapporoensis. These results suggest that high-throughput genotyping method can be used to gain more information regarding breeding systems and for more deeply understanding social evolution in the B. h. sapporoensis.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"3","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00009889279","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究ノート","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Notes","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11815191","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-3980","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"京都産業大学総合生命科学部"},{"subitem_text_value":"京都産業大学総合生命科学部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高橋, 純一","creatorNameLang":"ja"},{"creatorName":"TAKAHASHI, Jun-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"野村, 哲郎","creatorNameLang":"ja"},{"creatorName":"NOMURA, Tetsuro","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00009889279.pdf","filesize":[{"value":"313.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009889279.pdf","url":"https://ksu.repo.nii.ac.jp/record/2641/files/KJ00009889279.pdf"},"version_id":"6465c3d3-8845-4047-8e94-1ad9eb89d20b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"エゾオオマルハナバチ","subitem_subject_scheme":"Other"},{"subitem_subject":"マイクロサテライトDNA","subitem_subject_scheme":"Other"},{"subitem_subject":"マルチプレックスPCR","subitem_subject_scheme":"Other"},{"subitem_subject":"受精嚢内PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"マルハナバチ","subitem_subject_scheme":"Other"},{"subitem_subject":"Bombus hypocrita","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"multiplex PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"spermathecae PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"microsatellite DNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"bumblebee","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"エゾオオマルハナバチにおけるマイクロサテライトDNA ツールキット","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"エゾオオマルハナバチにおけるマイクロサテライトDNA ツールキット","subitem_title_language":"ja"},{"subitem_title":"A microsatellite DNA toolkit for genotyping of bumblebee Bombus hypocrita sapporoensis using the multiplex PCR","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["193"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2641","relation_version_is_last":true,"title":["エゾオオマルハナバチにおけるマイクロサテライトDNA ツールキット"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-27T01:41:27.770067+00:00"}