{"created":"2023-05-15T14:42:22.689574+00:00","id":2644,"links":{},"metadata":{"_buckets":{"deposit":"c12ba261-c803-4937-bd3f-9f698bae3fbb"},"_deposit":{"created_by":3,"id":"2644","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2644"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002644","sets":["14:179:193"]},"author_link":["7033","22301"],"control_number":"2644","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"70","bibliographicPageStart":"65","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"京都産業大学先端科学技術研究所所報"},{"bibliographic_title":"The bulletin of the Research Institute of Advanced Technology Kyoto Sangyo University","bibliographic_titleLang":"en"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"難治性末梢血管疾患の治療法として、自己の骨髄幹細胞を患部に移植して血管再生を促す治療が注目されている。幹細胞移植を効果的な血管再生療法として展開していくためには、発生過程における血管形成機構を分子・細胞レベルで詳細に解析し、機能する血管を再生するための技術的基盤を確立することが求められる。閉塞した冠動脈の機能を補う治療法の一つとして、血管を新たに形成するというアプローチが考えられる。心臓の表面を覆う心外膜は、主に未分化な増殖細胞から成り、冠動脈の全ての構成細胞すなわち内皮、平滑筋、線維芽細胞の前駆細胞を含んでいる。このことから、心臓幹細胞の重要な起源の一つと考えられている。そこで今回我々は、ニワトリ胚を用いて、心外膜原基の細胞移動や分化に影響を与えることが知られているBMP2、VEGF-A、PDGF-BB、FGF2、TGF-β1、TGF-β2 といった成長因子について、胚体内での作用を明らかにするため、遺伝子導入による強制発現を行った。その結果、VEGF-Aが発生過程の心臓に異所的な血管形成を引き起こすユニークな活性をもつことが明らかになった。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Autologous transplantation of bone marrow-derived stem cells is now a promising new type of therapy for peripheral vascular diseases. In order to properly develop this therapy as an effective treatment for peripheral vascular diseases, investigation of molecular, cellular mechanisms underlying vascular development and establishing a technical basis for the regeneration of functional vasculatures are longed for. The epicardium, the outermost layer of the heart, has been proposed to be an important origin of cardiac stem cells, which can differentiate into coronary vessels. It remains unclear, however, how they differentiate and coalesce into blood vessels in regionally restricted manners within the embryonic heart.Here, we show that in vivo expression of vascular endothelial growth factor (VEGF)-A in the epicardium of the avian embryonic heart promotes migration of epicardial cells and ectopic formation of blood vessel-like cysts surrounded by the endothelial and smooth muscle layers.Bmp2, PDGF-BB, FGF2, TGF- β 1 and TGF- β 2 showed no obvious effect. Thus, VEGF-A has a distinct activity to modulate coronary vascular patterns by promoting migration and differentiation of epicardial cells.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"6","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00009889339","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究活動報告","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Activity Reports","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11815191","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-3980","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"京都産業大学総合生命科学部生命システム学科"},{"subitem_text_value":"京都産業大学総合生命科学部生命システム学科"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石井, 泰雄","creatorNameLang":"ja"},{"creatorName":"ISHII, Yasuo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"板野, 直樹","creatorNameLang":"ja"},{"creatorName":"ITANO, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00009889339.pdf","filesize":[{"value":"301.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009889339.pdf","url":"https://ksu.repo.nii.ac.jp/record/2644/files/KJ00009889339.pdf"},"version_id":"3e55855d-7ba5-4d31-949b-d7b7da25090f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF-A","subitem_subject_scheme":"Other"},{"subitem_subject":"心外膜","subitem_subject_scheme":"Other"},{"subitem_subject":"冠動脈","subitem_subject_scheme":"Other"},{"subitem_subject":"心臓","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"VEGF","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"epicardium","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"coronary artery","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"heart","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"幹細胞ニッチの形成機構解明と血管再生療法への応用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"幹細胞ニッチの形成機構解明と血管再生療法への応用","subitem_title_language":"ja"},{"subitem_title":"Stem cell niche formation and its application to vascular reconstitution","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["193"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2644","relation_version_is_last":true,"title":["幹細胞ニッチの形成機構解明と血管再生療法への応用"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-27T01:53:39.670949+00:00"}