@article{oai:ksu.repo.nii.ac.jp:00002934,
 author = {横山, 謙 and YOKOYAMA, Ken and 中西, 温子 and NAKANISHI, Atsuko and 岸川, 淳一 and KISHIKAWA, Jun-ichi},
 journal = {京都産業大学総合学術研究所所報},
 month = {Jul},
 note = {液胞型ATPase V_0V_1およびその可溶性部分V_0V_1の構造決定を試みた。V_0V_1全体およびV_1部分のX線結晶構造解析用の結晶を作成した。高分解能の反射データを得るために，現在結晶を大きくするなどの条件検討を行っている。平行して凍結電子顕微鏡（クライオEM）を用いたV₁およびV_0V_1の単粒子解析を行った。V_1に関しては，良好な単粒子像が得られ，構造解析ソフトrelionで解析した結果，～9Å分解能の三次元構造を得た。また，界面活性剤を検討することで，解析に適したV_0V_1の画像が得られた。十分な枚数の画像を撮影し，いまだ明らかにされていないV_0V_1の原子分解能構造を決定する。, Vacuolar type ATPases（V_0V_1）are widely distributed in organisms and function as a proton pump responsible for acidification of intracellular compartments such as a lysosome, Golgi apparatus, endosome so on. In this study we have attempted to determine the structure of V_0V_1 and V_1 moiety. We obtained crystal of both the V_0V_1 and V_1 in several conditions. We are now carrying out the optimization of these crystal conditions. In addition, we observed the molecules of V_0V_1 and V_1 by cryo electron microscopy. Single particle analysis of the V_1 revealed 3-D averaged image at ~9 Å resolution. Also we obtained 2-D averaged images of V_0V_1 in which two stalks structure was clearly observed. These structural analysis of the V_0V_1 and V_1 would shed light on the molecular mechanism of V-ATPases in near future., 6, KJ00010262459, P, 研究論文, Papers},
 pages = {99--104},
 title = {液胞型ATPase(V_0V_1)の構造解析},
 volume = {11},
 year = {2016}
}