{"created":"2023-05-15T14:42:36.003761+00:00","id":2934,"links":{},"metadata":{"_buckets":{"deposit":"ce42ca58-55cc-44cf-b0b8-2c2ac793cdc0"},"_deposit":{"created_by":3,"id":"2934","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2934"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002934","sets":["14:226:227"]},"author_link":["21613","21612","21611"],"control_number":"2934","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"104","bibliographicPageStart":"99","bibliographicVolumeNumber":"11","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"液胞型ATPase V_0V_1およびその可溶性部分V_0V_1の構造決定を試みた。V_0V_1全体およびV_1部分のX線結晶構造解析用の結晶を作成した。高分解能の反射データを得るために，現在結晶を大きくするなどの条件検討を行っている。平行して凍結電子顕微鏡（クライオEM）を用いたV₁およびV_0V_1の単粒子解析を行った。V_1に関しては，良好な単粒子像が得られ，構造解析ソフトrelionで解析した結果，～9Å分解能の三次元構造を得た。また，界面活性剤を検討することで，解析に適したV_0V_1の画像が得られた。十分な枚数の画像を撮影し，いまだ明らかにされていないV_0V_1の原子分解能構造を決定する。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Vacuolar type ATPases（V_0V_1）are widely distributed in organisms and function as a proton pump responsible for acidification of intracellular compartments such as a lysosome, Golgi apparatus, endosome so on. In this study we have attempted to determine the structure of V_0V_1 and V_1 moiety. We obtained crystal of both the V_0V_1 and V_1 in several conditions. We are now carrying out the optimization of these crystal conditions. In addition, we observed the molecules of V_0V_1 and V_1 by cryo electron microscopy. Single particle analysis of the V_1 revealed 3-D averaged image at ~9 Å resolution. Also we obtained 2-D averaged images of V_0V_1 in which two stalks structure was clearly observed. These structural analysis of the V_0V_1 and V_1 would shed light on the molecular mechanism of V-ATPases in near future.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"6","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00010262459","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究論文","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Papers","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"京都産業大学総合生命科学部"},{"subitem_text_value":"京都産業大学リエゾンオフィス"},{"subitem_text_value":"京都産業大学リエゾンオフィス"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"横山, 謙","creatorNameLang":"ja"},{"creatorName":"YOKOYAMA, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中西, 温子","creatorNameLang":"ja"},{"creatorName":"NAKANISHI, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岸川, 淳一","creatorNameLang":"ja"},{"creatorName":"KISHIKAWA, Jun-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00010262459.pdf","filesize":[{"value":"279.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00010262459.pdf","url":"https://ksu.repo.nii.ac.jp/record/2934/files/KJ00010262459.pdf"},"version_id":"92a44f14-8395-4c60-a985-5b7d72bcf4de"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"液胞型 ATPase","subitem_subject_scheme":"Other"},{"subitem_subject":"構造解析","subitem_subject_scheme":"Other"},{"subitem_subject":"V-ATPase","subitem_subject_scheme":"Other"},{"subitem_subject":"V_0V_1","subitem_subject_scheme":"Other"},{"subitem_subject":"電子顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"V-ATPase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rotary molecular motor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Structural study","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryo-electron micrography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"V_0V_1","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"液胞型ATPase(V_0V_1)の構造解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"液胞型ATPase(V_0V_1)の構造解析","subitem_title_language":"ja"},{"subitem_title":"Structural Studies of the Vacuolar type ATPase","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["227"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2934","relation_version_is_last":true,"title":["液胞型ATPase(V_0V_1)の構造解析"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-04T02:55:19.670236+00:00"}