{"created":"2023-05-15T14:42:37.232928+00:00","id":2962,"links":{},"metadata":{"_buckets":{"deposit":"1a02f0de-f892-4016-8423-501111905e0d"},"_deposit":{"created_by":3,"id":"2962","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2962"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002962","sets":["14:226:229"]},"author_link":["7670","7746","7745","7747","22307"],"control_number":"2962","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"94","bibliographicPageStart":"87","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"光合成の場である葉緑体にはDNAが存在し，核とは異なる独自のDNA複製機構を持っている。葉緑体DNAの複製機構には不明な点が多く，複製開始に必要なDNAプライマーゼも同定されていなかった。筆者らは，T7ファージのT7 bacteriophage gene 4 protein（T7gp4）というDNAヘリカーゼ／プライマーゼのホモログであるTWINKLEが，植物では葉緑体に局在してDNA複製を開始するDNAプライマーゼとして働いているのではないかと考えて研究を進めている。本研究では，パーティクルガン法によりTWINKLE-GFP融合蛋白質を一過的に植物細胞内で発現させることで細胞内局在解析をおこない，TWINKLE蛋白質が葉緑体に局在していることを明らかにした。この結果は，植物のTWINKLEが葉緑体DNAの複製に働くDNAプライマーゼであることを強く示唆するものである。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Plastids are major organelles found in plant cells and contain their own genomes. Plastids are thought to have unique DNA replication systems, where DNA primase and DNA helicases are responsible for synthesis of primers and DNA unwinding, respectively. While earlier studies have identified DNA primase and helicase activities in a plastid fraction but little is known about genes encoding the plastidial DNA pirmase. Here we characterized a rice homologue of TWINKLE（Oryza sativa cv. Nipponbare, termed OsTWINKLE）, which is an autosomal dominant progressive external ophthalmoplegia（adPEO）-associated gene that encodes mitochondrial DNA helicase in mammalian cells. Subcellular localization analyses using GFP fusion protein showed that OsTWINKLE is distributed in plastids.These results suggested that the plant homologue of TWINKLE functions as a DNA primase during plastidial DNA replication.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"6","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00009319259","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究論文","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Paper","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"東京理科大学理工学部"},{"subitem_text_value":"東京理科大学理工学部"},{"subitem_text_value":"東京理科大学総合研究機構"},{"subitem_text_value":"東京理科大学総合研究機構"},{"subitem_text_value":"京都産業大学総合生命科学部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"稲垣, 麻美","creatorNameLang":"ja"},{"creatorName":"INAGAKI, Asami","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"内山, 幸伸","creatorNameLang":"ja"},{"creatorName":"UCHIYAMA, Yukinobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金井, 良博","creatorNameLang":"ja"},{"creatorName":"KANAI, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坂口, 謙吾","creatorNameLang":"ja"},{"creatorName":"SAKAGUCHI, Kengo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木村, 成介","creatorNameLang":"ja"},{"creatorName":"KIMURA, Seisuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00009319259.pdf","filesize":[{"value":"312.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009319259.pdf","url":"https://ksu.repo.nii.ac.jp/record/2962/files/KJ00009319259.pdf"},"version_id":"b4cdf414-83d1-434c-a734-2a333c37c229"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"葉緑体","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA プライマーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA ヘリカーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"TWINKLE","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA 複製","subitem_subject_scheme":"Other"},{"subitem_subject":"Plastid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA primase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA helicase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TWINKLE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA replication","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : 細胞内局在の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : 細胞内局在の検討","subitem_title_language":"ja"}]},"item_type_id":"2","owner":"3","path":["229"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2962","relation_version_is_last":true,"title":["植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : 細胞内局在の検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-04T06:40:10.921282+00:00"}