{"created":"2023-05-15T14:42:37.912171+00:00","id":2978,"links":{},"metadata":{"_buckets":{"deposit":"87cb385f-4841-4e58-a5fc-05be0a0111b5"},"_deposit":{"created_by":3,"id":"2978","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2978"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002978","sets":["14:226:229"]},"author_link":["22047","7727"],"control_number":"2978","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"241","bibliographicPageStart":"235","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"アフリカツメガエルXenopus laevis卵の受精成立にかかわる分子機構を明らかとすることを目的として，卵の細胞膜マイクロドメイン（MD)およびMD局在分子の生理機能に関する研究を行った。その結果，未受精卵から単離したMD画分には精子との相互作用能とそのことによる受精シグナル伝達反応（精子依存的なチロシンキナーゼSrcの活性化，ウロプラキンIIIの部分消化とチロシンリン酸化) の再構成能があること，そしてこの未受精卵MDの機能は卵巣内で実行されるホルモン依存的な卵成熟反応にともない構築されることを明らかにした。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"In the African clawed frog Xenopus laevis, sperm-egg interaction promotes partial proteolysis and/or tyrosine phosphorylation of uroplakin III（UPIII) and the tyrosine kinase Src（Src) , both of which localize to the cholesterol-enriched egg membrane microdomains（MDs) . Here we show that sperm promote the proteolysis and/or tyrosine phosphorylation of UPIII and Src in the MDs that are isolated from ovulated and unfertilized eggs（UF-MDs) . An antibody against the extracellular domain of UPIII interferes with these events. On the other hand, the inhibitory effect of anti-UPIII antibody on normal fertilization is canceled by co-incubation with UF-MDs. These results suggest that the isolated UF-MDs are capable of interacting with sperm as MDs in the intact eggs do. However, this interaction does not interfere with normal fertilization, but rather augments the ability of sperm to fertilize eggs that are pretreated with the anti-UPIII antibody. This unexpected effect of UF-MDs on sperm requires UPIII function in the UF-MDs and protein kinase activity in sperm. We also found that MDs that are isolated from progesterone-treated mature oocytes, but not ovarian immature oocytes, are similarly functional as UF-MDs. Taken together, we propose a model that structural and functional competency of the UPIII-Src signaling system in MDs is strictly regulated in the course of oocyte maturation and subsequently in sperm-mediated egg activation and fertilization.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"22","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00009321179","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究活動報告","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Activity Reports","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"京都産業大学総合生命科学部"},{"subitem_text_value":"京都産業大学総合生命科学部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"佐藤, 賢一","creatorNameLang":"ja"},{"creatorName":"SATO, Ken-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"井尻, 貴之","creatorNameLang":"ja"},{"creatorName":"IJIRI, Takashi W.","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00009321179.pdf","filesize":[{"value":"258.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00009321179.pdf","url":"https://ksu.repo.nii.ac.jp/record/2978/files/KJ00009321179.pdf"},"version_id":"63caafea-3ed2-4d86-a043-c39dcff5c934"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アフリカツメガエル","subitem_subject_scheme":"Other"},{"subitem_subject":"受精","subitem_subject_scheme":"Other"},{"subitem_subject":"配偶子間相互作用","subitem_subject_scheme":"Other"},{"subitem_subject":"卵成熟","subitem_subject_scheme":"Other"},{"subitem_subject":"膜マイクロドメイン","subitem_subject_scheme":"Other"},{"subitem_subject":"African clawed frog","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"fertilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gamete interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"membrane microdomains","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"oocyte maturation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"卵母細胞の形成と成熟に伴う膜マイクロドメインの機能構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"卵母細胞の形成と成熟に伴う膜マイクロドメインの機能構築","subitem_title_language":"ja"},{"subitem_title":"Egg membrane microdomains-associated uroplakin III-Src system becomes functional during oocyte maturation and is required for bidirectional gamete signaling at fertilization in the frog Xenopus laevis","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["229"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2978","relation_version_is_last":true,"title":["卵母細胞の形成と成熟に伴う膜マイクロドメインの機能構築"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-04T07:33:24.967684+00:00"}