{"created":"2023-05-15T14:42:38.166979+00:00","id":2984,"links":{},"metadata":{"_buckets":{"deposit":"cfc8b4b9-e6c3-43d0-b015-a221342977f7"},"_deposit":{"created_by":3,"id":"2984","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"2984"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00002984","sets":["14:226:230"]},"author_link":["7743","7744","7745","7746","7747","22307"],"control_number":"2984","item_2_biblio_info_12":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"55","bibliographicPageStart":"49","bibliographicVolumeNumber":"8","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_2_description_10":{"attribute_name":"抄録(日)","attribute_value_mlt":[{"subitem_description":"細胞内共生により生じた葉緑体は，核とは異なる独自のDNA複製機構を持っている。これまで，葉緑体DNAの複製開始に必要なDNAプライマーゼは同定されていなかった。筆者らは，T7ファージのT7 bacteriophage gene 4 protein（T7gp4）というDNAヘリカーゼ/プライマーゼのホモログであるTWINKLEが，植物では葉緑体に局在してDNA複製を開始するDNAプライマーゼとして働いているのではないかという仮説を立てて研究を進めている。本研究では，ホモロジーモデリングなどの手法によりT7gp4，動物および植物のTWINKLEのアミノ酸配列を比較し，植物ではDNAプライマーゼドメインが高度に保存されていることを明らかにした。この結果は，植物のTWINKLEが葉緑体DNAの複製に働くDNAプライマーゼであることを強く示唆する。","subitem_description_type":"Other"}]},"item_2_description_11":{"attribute_name":"抄録(英)","attribute_value_mlt":[{"subitem_description":"Plastids are major organelles found in plant cells and contain their own genomes. Plastids are thought to have unique DNA replication systems, where DNA primase and DNA helicases are responsible for synthesis of primers and DNA unwinding, respectively. While earlier studies have identified DNA primase and helicase activities in a plastid fraction but little is known about genes encoding the plastidial DNA pirmase and DNA helicase.\n　In animal cells, TWINKLE protein, which is is homologue of bacteriophage T7gp4, is known to be localized to mitochondria. While T7gp4 has both DNA primase and helicase activities, animal TWINLE protein has only DNA helicase activity due to sequence change in DNA primase domain. Plant genome encodes TWINKLE protein, and our preliminary subcellular localization analysis showed that rice TWINLE protein is targeted to plastid. Amino acid sequence comparison and homology modeling analysis showed that the plant TWINKLE proteins retain sequence homology for DNA primase domain of the T7gp4. These results suggested that the plant homologue of TWINKLE functions as a DNA primase and DNA helicase during plastidial DNA replication.","subitem_description_type":"Other"}]},"item_2_description_15":{"attribute_name":"表示順","attribute_value_mlt":[{"subitem_description":"5","subitem_description_type":"Other"}]},"item_2_description_16":{"attribute_name":"アクセション番号","attribute_value_mlt":[{"subitem_description":"KJ00008753359","subitem_description_type":"Other"}]},"item_2_description_2":{"attribute_name":"ページ属性","attribute_value_mlt":[{"subitem_description":"P","subitem_description_type":"Other"}]},"item_2_description_8":{"attribute_name":"記事種別(日)","attribute_value_mlt":[{"subitem_description":"研究論文","subitem_description_type":"Other"}]},"item_2_description_9":{"attribute_name":"記事種別(英)","attribute_value_mlt":[{"subitem_description":"Paper","subitem_description_type":"Other"}]},"item_2_source_id_1":{"attribute_name":"雑誌書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_2_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_2_text_6":{"attribute_name":"著者所属(日)","attribute_value_mlt":[{"subitem_text_value":"Division of Basic Sciences, Fred Hutchinson Cancer Research Center"},{"subitem_text_value":"京都産業大学総合生命科学部"},{"subitem_text_value":"東京理科大学総合研究機構"},{"subitem_text_value":"東京理科大学理工学部"},{"subitem_text_value":"東京理科大学総合研究機構"},{"subitem_text_value":"京都産業大学総合生命科学部"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"武内, 亮","creatorNameLang":"ja"},{"creatorName":"タケウチ, リョウ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKEUCHI, Ryo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中山, 北斗","creatorNameLang":"ja"},{"creatorName":"ナカヤマ, ホクト","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAYAMA, Hokuto","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"金井, 良博","creatorNameLang":"ja"},{"creatorName":"カナイ, ヨシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"KANAI, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"内山, 幸伸","creatorNameLang":"ja"},{"creatorName":"ウチヤマ, ユキノブ","creatorNameLang":"ja-Kana"},{"creatorName":"UCHIYAMA, Yukinobu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坂口, 謙吾","creatorNameLang":"ja"},{"creatorName":"サカグチ, ケンゴ","creatorNameLang":"ja-Kana"},{"creatorName":"SAKAGUCHI, Kengo","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木村, 成介","creatorNameLang":"ja"},{"creatorName":"キムラ, セイスケ","creatorNameLang":"ja-Kana"},{"creatorName":"KIMURA, Seisuke","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-28"}],"displaytype":"detail","filename":"KJ00008753359.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KJ00008753359.pdf","url":"https://ksu.repo.nii.ac.jp/record/2984/files/KJ00008753359.pdf"},"version_id":"f9807972-fc2d-4254-aad8-fdb73461d987"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"葉緑体","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA プライマーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA ヘリカーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"TWINKLE","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA 複製","subitem_subject_scheme":"Other"},{"subitem_subject":"Plastid","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA primase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA helicase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TWINKLE","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA replication","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : アミノ酸配列の比較による検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : アミノ酸配列の比較による検討","subitem_title_language":"ja"},{"subitem_title":"Homology modeling suggested that plant TWINKLE protein has both DNA primase and DNA helicase activities and may functions as plastidial DNA replication enzyme","subitem_title_language":"en"},{"subitem_title":"ショクブツ ノ TWINKLE ハ ヨウリョクタイ DNA フクセイ ニ カカワル DNA プライマーゼ カ ? アミノ サン ハイレツ ノ ヒカク ニヨル ケントウ","subitem_title_language":"ja-Kana"}]},"item_type_id":"2","owner":"3","path":["230"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-01-28"},"publish_date":"2018-01-28","publish_status":"0","recid":"2984","relation_version_is_last":true,"title":["植物のTWINKLEは葉緑体DNA複製に関わるDNAプライマーゼか? : アミノ酸配列の比較による検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-06T05:36:02.458772+00:00"}