{"created":"2023-05-15T14:46:25.597915+00:00","id":9929,"links":{},"metadata":{"_buckets":{"deposit":"30221558-1e47-4209-84e6-d30482f62244"},"_deposit":{"created_by":11,"id":"9929","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"9929"},"status":"published"},"_oai":{"id":"oai:ksu.repo.nii.ac.jp:00009929","sets":["14:226:961"]},"author_link":["21611","21612","21613"],"control_number":"9929","item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"52","bibliographicPageStart":"47","bibliographicVolumeNumber":"12","bibliographic_titles":[{"bibliographic_title":"京都産業大学総合学術研究所所報"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"クライオ電子顕微鏡を用いた単粒子解析により液胞型 ATPase（V_0V_1）の全体構造の解明を試みた。V_0V_1 を含む試料溶液を観察用グリッド上に添加し，急速凍結することで，電子線が透過できるクライオグリッドを作成した。界面活性剤の濃度を減らすことで良好なグリッドが作成でき，そこから数千枚の電子顕微鏡画像を得ることができた。構造解析ソフトにより，単粒子画像をピックアップし，平均化することで三次元再構成による立体構造の再構成が可能になった。解析を進めることで，V_0V_1の立体構造を決定し，その分子機構を明らかにする道筋がついた。","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"京都産業大学総合学術研究所"}]},"item_10002_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AA11879037","subitem_source_identifier_type":"NCID"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1348-8465","subitem_source_identifier_type":"PISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岸川, 淳一","creatorNameLang":"ja"},{"creatorName":"KISHIKAWA, Jun-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中西, 温子","creatorNameLang":"ja"},{"creatorName":"NAKANISHI, Atsuko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"横山, 謙","creatorNameLang":"ja"},{"creatorName":"YOKOYAMA, Ken","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-24"}],"displaytype":"detail","filename":"KSUSGKS_12_47.pdf","filesize":[{"value":"325.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KSUSGKS_12_47.pdf","url":"https://ksu.repo.nii.ac.jp/record/9929/files/KSUSGKS_12_47.pdf"},"version_id":"24b71082-158a-41d7-8554-95549632f1e7"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"液胞型 ATPase","subitem_subject_scheme":"Other"},{"subitem_subject":"構造解析","subitem_subject_scheme":"Other"},{"subitem_subject":"クライオ電子顕微鏡","subitem_subject_scheme":"Other"},{"subitem_subject":"単粒子解析","subitem_subject_scheme":"Other"},{"subitem_subject":"回転分子モーター","subitem_subject_scheme":"Other"},{"subitem_subject":"V-ATPase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rotary molecular motor","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Structural study","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryo-electron micrography","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Single particle analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"クライオ電子顕微鏡による液胞型ATPase（V_0V_1）の構造解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"クライオ電子顕微鏡による液胞型ATPase（V_0V_1）の構造解析","subitem_title_language":"ja"},{"subitem_title":"Structural analysis of Vacuolar type ATPase（ V_0V_1）using cryo electron microscope","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["961"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-02-24"},"publish_date":"2018-02-24","publish_status":"0","recid":"9929","relation_version_is_last":true,"title":["クライオ電子顕微鏡による液胞型ATPase（V_0V_1）の構造解析"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-08-31T06:25:18.767234+00:00"}