Kyoto is one of the historically important and famous international cities for site-seeing. Every year, many visitors come to Kyoto City from all over the world. Once a certain infectious disease is imported into Kyoto City from visitor’s home town in which the outbreak of the disease occur, the disease might be spread rapidly to whole the city and even to other countries. Especially, arthropodborne diseases have been a world public health problem because of on going global warming. In this study, we conducted vector surveillance for mosquito-borne diseases at a temple “K” located at urban area in Kyoto City, from May, 2013 to March, 2014. Mosquitoes were captured using CDC-dry ice trap for night-active mosquitoes, and using human-trap for day-time active ones. We captured 1,407 mosquitoes belonging to three genera including 5 species, Aedes(Ae.)albopictus(Skuse)(53%), Ae.japonicas(Theobald)(0.2%), Culex(Cx.)pipiens complex(46.1%), Cx. tritaeniorhynchus(Giles)(0.4%),and Armigeres subalbatus(Coquillett)(0.3%). We genetically classified Cx. pipiens complex further into Cx. pipience pallens(Coquillett)(around 95%)and Cx. pipiens form molestus(Forskal)(around 3%). Ae. albopictus(Skuse)was predominant among mosquitoes captured as day-time active ones. The population peak of Ae. albopictus(Skuse)during research period, was June, 2013. Cx. pipiens complex and Ae. albopictus(Skuse)were predominant species as night-time active ones. The population peak of Cx. pipiens complex and Ae. albopictus(Skuse)were June and August, 2013, respectively.